Apotransferrin and Holotransferrin Undergo Different Endocytic Cycles in Intestinal Epithelia (Caco-2) Cells
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چکیده
منابع مشابه
The effect of apotransferrin on iron release from Caco-2 cells, an intestinal epithelial cell line.
The Caco-2 cell line grown in bicameral chambers was used to study the effect of transferrin in the basal chamber on the transepithelial transport of iron. We have shown that when iron was offered as 59Fe on the apical surface of the Caco-2 cells, transport of 59Fe into the basal chamber was stimulated by 50 micromol/L apotransferrin. Here, we examined the effect on 59Fe transport of lower conc...
متن کاملVesicular transport and apotransferrin in intestinal iron absorption, as shown in the Caco-2 cell model.
The potential roles of vesicular transport and apotransferrin (entering from the blood) in intestinal Fe absorption were investigated using Caco-2 cell monolayers with tight junctions in bicameral chambers as a model. As shown previously, addition of 39 microM apotransferrin (apoTf) to the basolateral fluid during absorption studies markedly stimulated overall transport of 1 microM (59)Fe from ...
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The human colonic adenocarcinoma derived cell line, Caco-2 [l], may be grown as a polarized epithelium on permeable filter supports and develops a number of characteristic enterocyte functions when grown to confluency. The differentiated functions include the development of apical brush border microvilli, the presence of tight junctions, the unique electrical properties of transporting epitheli...
متن کاملEndocytic pathway of exogenous iron-loaded ferritin in intestinal epithelial (Caco-2) cells.
Ferritin, a food constituent of animal and vegetal origin, is a source of dietary iron. Its hollow central cavity has the capacity to store up to 4,500 atoms of iron, so its potential as an iron donor is advantageous to heme iron, present in animal meats and inorganic iron of mineral or vegetal origin. In intestinal cells, ferritin internalization by endocytosis results in the release of its ir...
متن کاملUptake of different crystal structures of TiO₂ nanoparticles by Caco-2 intestinal cells.
The gastrointestinal uptake of different crystal structures of TiO2 was investigated using Caco-2 intestinal cells. Caco-2 monolayers exhibited time-dependent, saturable uptake of Ti from TiO2 exposures of 1 mgl(-1) over 24h, which was influenced by crystal type. Initial uptake rates were 5.3, 3.73, 3.58 and 4.48 nmol mg(-1)protein h(-1) for bulk, P25, anatase and rutile forms respectively. All...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1997
ISSN: 0021-9258
DOI: 10.1074/jbc.272.31.19425